Tuesday, 29 June 2010

AOAC Official Method 2000.03 Ochratoxin A in Barley Immunoaffinity by Column HPLC First Action 2000

(Applicable to the determination of ochratoxin A in barley at >1 ng/g)
See Table 2000.03A for results of the interlaboratory study supporting the acceptance of the method.

A. Principle

Test portion is extracted by blending with acetonitrile-water. The extract is cleaned up by passing through an immunoaffinity column. Ochratoxin A (OTA) is eluted with methanol, further purifed and identified by LC, and quantified by fluorescence.
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2 comments:

  1. Joyce23 April 2020 at 03:21

    The units for OTA are mcg/g at the calculation for the standard solution. Is this correct? Should the units be mcg/mL?

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  2. Unknown23 July 2020 at 23:34

    In standard solution using solvent or matrix extract will be in be mcg/ml but calculation for sample concentration should be mcg/g

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